The worth of creating extensive, tissue-specific maps of protein lysine acetylation sites is additional underscored by the numerous function of posttranslational modifications within the evolution and phenotypic composition of vertebrates, as indicated by the significant selective strain gene regulatory parts near posttranslational-modifying enzymes have come below in placental animals ( Lowe et al., 2011 ). Right here, lawyer we combined an environment friendly method for protein extraction from tissue samples with lysine-acetylated peptide immunoprecipitation and excessive-accuracy tandem mass spectrometric (MS/MS) measurements, which allowed us to generate an atlas of lysine acetylation websites in 16 completely different rat tissues.

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