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They found that no regular cerevisiae or carlsbergensis strains utilised lysine whereas many different yeasts, together with wild yeasts, did so. They saved their inventory cultures on malt extract agar slopes or on malt extract chalk agar within the case of Brettanomyces species. Later, Morris & Eddy1 described a stable lysine medium for the isolation and enumeration of untamed yeasts in pitching yeast.

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